After years of being sheltered from the human world, the Turtle brothers Donatello (Micah Abbey), Michelangelo (Shamon Brown Jr.), Leonardo (Nicolas Cantu), and Raphael (Brady Noon) decide to win the hearts of New Yorkers and be accepted as normal teenagers through heroic acts.

They set out to defeat villain Superfly (Ice Cube), a faceless antagonist who has been killing people across New York. Their new friend April O'Neil (Ayo Edebiri) helps them take on a mysterious crime syndicate, but they soon get in over their heads when an army of mutants is unleashed upon them. Believing that mutants belong underground and away from society, they want to rewrite this narrative with karate, friendship, and pizza.